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HLA B-27, Flowcytometry

  • You need to provide Blood
  • Overnight fasting is not mandatory
  • This test is for Male, Female


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SKU: FPE2568569545 Categories: , Tags: , ,


What is HLA B-27, Flowcytometry?

HLA B-27, Flow Cytometry test is performed to measure the level of HLA B27 antigen in blood. Presence of HLA B-27 may indicate a possibility of autoimmune disease, where the guarding cells (WBC) attacks own cells.
This test is done if one experiences signs and symptoms of arthritis such as chronic joint pain, inflammation in the lower back and hips. It can also help detect inflammatory bowel disease and other chronic conditions.
People with a positive HLA B-27 have an increased chance of developing autoimmune diseases like rheumatoid arthritis, juvenile idiopathic arthritis, and ankylosing spondylitis.

Why is HLA B-27, Flowcytometry done?

The HLA B-27 test is done:

  • In case of symptoms of arthritis such as chronic joint pain, stiffness, and inflammation in the lower back, hips, neck, and chest
  • In the case of Uveitis (painful inflammation of eyes) especially in males and of the early 30s age group.
What does HLA B-27, Flowcytometry Measure?

HLA B-27 test determines the presence or absence of human leukocyte antigen which is present on the surface of white blood cells. The HLA B-27 also refers to the gene which codes for the HLA B-27 protein.

HLA B-27 are proteins which help the immune system to differentiate the body’s own cells from other foreign cells. Every person has an inherited combination of HLA genes which codes for many antigens which are present on the surfaces of their cells. This HLA combination is distinctive for each person and is based on the presence or absence of each antigen.

People with HLA B-27 have increased chances of developing autoimmune diseases like ankylosing spondylitis, rheumatoid arthritis, reactive arthritis, and isolated acute anterior uveitis. HLA B-27 is also said to be linked with inflammatory bowel disease and other chronic conditions.

Interpreting HLA B-27, Flowcytometry results

Negative No expression of HLA B-27
Indeterminate Grey zone (within 10 channels of cutoff)
Positive Expression of HLA B-27 detected

Anti-HLA-B27 antibody cross reacts with several other antigens of HLA-B group specially HLA-B7 leading to false-positive results*

Additional information


Healths First, Lal Pathlab, Medcis Pathlab


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